Anti-Thiophosphate ester antibody [51-8] ab92570 from Abcam

Product Specs
ItemAnti-Thiophosphate ester antibody [51-8]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab92570
Domain/Region/Terminus51-8
Clone51-8
HostRabbit
Antibody TypePrimary
ApplicationsWestern blot
Conjugate/TagUnconjugated
Quantity50 UL
IsotypeIgG
ClonalityMonoclonal
AntigenThiophosphate ester
ReactivityAll

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Cyclin D1 extensively reprograms metabolism to support biosynthetic pathways in hepatocytesJournal of Biological ChemistryDecember 1, 2023Heng Wu, Betsy T. Kren, Andrew N. Lane, Teresa A. Cassel, Richard M. Higashi, Teresa W.M. Fan, George S. Scaria, Laurie L. Shekels, Mark A. Klein, Jeffrey H. Albrechtroom temperature. This was run for Western blot as above, and using anti-thiophosphate ester antibody (ab92570, Abcam). Using a previously described FLAG-HA-CAD plasmid construct (43) (obtained from Addgene), an

(2) IL-6/JAK2-dependent G6PD phosphorylation promotes nucleotide synthesis and supports tumor growthMolecular MetabolismDecember 1, 2023Xuemei Qiu, Hongping Ye, Xiaofei Li, Dan Li, Lu Jiang, Rui Liu, Zhe Zhao, Dan HeSDS-PAGE separation, followed by immunoblot analysis with the thiophosphate ester specific antibody (ab92570, Abcam). 2.10 Analyses of protein structure The PyMOL software was used to analyzed the protein structure

(3) Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate CancerAdvanced ScienceNovember 10, 2023Huaiyuan Liang, Chunguang Yang, Ruijiang Zeng, Yingqiu Song, Jianxi Wang, Wei Xiong, Binyuan Yan, Xin Jinroom temperature for 1 h. The phosphorylated protein was detected by thiophosphate ester antibody (# ab92570, Abcam). For PLK1 kinase assay in cells, the C4‐2 cells were transfected with indicated constructs or

(4) Atypical Protein Kinase C Promotes its own Asymmetric Localisation by Phosphorylating Cdc42 in Polarising CellsbioRxivNovember 2, 2023John Packer, Alicia G. Gubieda, Aaron Brooks, Lars N. Deutz, Iolo Squires, Shona Ellison, Sundar Ram Naganathan, Adam J.M. Wollman, Daniel J. Dickinson, Josana Rodriguezprocessed for Western-blot and phosphorylation was detected using an anti-thiophosphate ester antibody (#ab92570, Abcam). In vitro – Purification of CDC-42 recombinant proteins: MBP::CDC-42 and MBP::CDC-42(S71A)

(5) DNA methylation-dependent epigenetic regulation of Verticillium dahliae virulence in plantsaBIOTECHSeptember 20, 2023Yun-Ya Chen, Chen Zhu, Jian-Hua Zhao, Ting Liu, Feng Gao, Ying-Chao Zhang, Cheng-Guo Duangel was transferred to a PVDF membrane for western blots. Anti-thiophosphate ester antibody (Abcam, ab92570) was used to detect phosphorylated proteins. Chromatin immunoprecipitation ChIP assay was performed

(6) Rapid phosphorylation of glucose-6-phosphate dehydrogenase by casein kinase 2 sustains redox homeostasis under ionizing radiationRedox BiologySeptember 1, 2023Yilong Hao, Tao Ren, Xiaoke Huang, Mi Li, Jong-Ho Lee, Qianming Chen, Rui Liu, Qingfeng Tangprecipitated and analyzed by SDS-PAGE, followed by immunoblot with the thiophosphate ester specific antibody (ab92570, Abcam). 2.9 Measurement of G6PD binding with NADP+ Cellular G6PD proteins or bacterially purified

(7) Endothelial RIPK1 protects artery bypass graft against arteriosclerosis by regulating SMC growthScience AdvancesSeptember 1, 2023Yao Lu, Yiming Leng, Yalan Li, Jie Wang, Wei Wang, Ruilin Wang, Yuanyuan Liu, Qian Tan, Wenjing Yang, Youxiang Jiang, et al.resolved by SDS-PAGE. Phosphorylation by RIPK1 was detected by anti–thiophosphate ester antibody (Abcam, ab92570). In vitro methylation assays To test the impact of EEF1AKMT3 phosphorylation on EEF1A, the direct

(8) Gasdermin D-mediated pyroptosis is regulated by AMPK-mediated phosphorylation in tumor cellsCell death & diseaseJuly 26, 2023Chu X, Xiao X, Wang G, Uosef A, Lou X, Arnold P, Wang Y, Kong G, Wen M, Minze LJ, et al.buffer and resolved by SDS–PAGE. Phosphorylation of GD-NT was detected using anti-Thiophosphate ester (ab92570, Abcam). In regular ATP-containing kinase reaction, 1 μg of GST-GD-NT purified from HEK293 cells transfected

(9) CDK12 and Integrator-PP2A complex modulates LEO1 phosphorylation for processive transcription elongationScience advancesMay 19, 2023Qiu M, Yin Z, Wang H, Lei L, Li C, Cui Y, Dai R, Yang P, Xiang Y, Li Q, et al.II Rpb1 NTD (D8L4Y) rabbit mAb (anti-POLR2A, catalog no. 14958) and anti-ThioP antibody (catalog no. ab92570) were obtained from Abcam. Monoclonal anti-FLAG M2 antibody (catalog no. F3165) and anti–β-tubulin (C66)

(10) ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapyNature communicationsMay 19, 2023Xiao X, Shi J, He C, Bu X, Sun Y, Gao M, Xiang B, Xiong W, Dai P, Mao Q, et al.the signal of phosphorylation was detected by immunoblotting with anti-thiophosphate ester antibody (ab92570, Abcam). MS analysis for identifying phosphorylation on PD-1 The coomassie-stained SDS-PAGE gel pieces

(11) In vitro kinase assay reveals ADP-heptose-dependent ALPK1 autophosphorylation and altered kinase activity of disease-associated ALPK1 mutantsScientific reportsApril 18, 2023García-Weber D, Dangeard AS, Teixeira V, Hauke M, Carreaux A, Josenhans C, Arrieumerlou C.anti-GST antibody (Abcam, ab19256) and a rabbit polyclonal anti-thiophosphate ester antibody (Abcam, ab92570), respectively. HRP-conjugated secondary antibodies were purchased from GE Healthcare, Cell Signaling

(12) Branch point strength controls species-specific CAMK2B alternative splicing and regulates LTPLife Science AllianceMarch 1, 2023Andreas Franz, A Ioana Weber, Marco Preußner, Nicole Dimos, Alexander Stumpf, Yanlong Ji, Laura Moreno-Velasquez, Anne Voigt, Frederic Schulz, Alexander Neumann, et al.SDS–PAGE and semi-dry Western blotting. The blot was developed using an anti-thiophosphate ester antibody (ab92570; Abcam) and an HRP-linked anti-rabbit antibody (Cell Signaling Technologies). For thiophosphate enrichment,

(13) Loss of NDR1/2 kinases impairs endomembrane trafficking and autophagy leading to neurodegenerationLife science allianceFebruary 1, 2023Roşianu F, Mihaylov SR, Eder N, Martiniuc A, Claxton S, Flynn HR, Jalal S, Domart MC, Collinson L, Skehel M, et al.(1:1,000, ab106269; Abcam), rabbit Raph1 (1:1,000, ab121619; Abcam) and rabbit thiophosphate ester (1:200, ab92570; Abcam). The Raph1 phospho-S192 antibody is a custom-made rabbit polyclonal antibody raised against

(14) Reciprocal inhibition of PIN1 and APC/C(CDH1) controls timely G1/S transition and creates therapeutic vulnerabilityResearch squareJanuary 19, 2023Ke S, Dang F, Wang L, Chen JY, Naik MT, Thavamani A, Liu Y, Li W, Kim N, Naik NM, et al.boiled for 10 min. Samples were then subjected to IB using anti-Thiophosphate ester antibody (Abcam, ab92570). In vivo therapy for patient-derived xenografts All animal experiments were approved by the IACUC

(15) Molecular mechanism of toxin neutralization in the HipBST toxin-antitoxin system of Legionella pneumophilaNature CommunicationsDecember 1, 2022Xiangkai Zhen, Yongyu Wu, Jinli Ge, Jiaqi Fu, Le Ye, Niannian Lin, Zhijie Huang, Zihe Liu, Zhao-qing Luo, Jiazhang Qiu, et al.overnight at 4 °C with a primary antibody against thiophosphate ester (rabbit anti-thiophosphate ester (ab92570), Abcam, dilution 1:4000 v/v). The membrane was then washed three times with TBST before being incubated

(16) Dichotomy of the BSL phosphatase signaling spatially regulates MAPK components in stomatal fate determinationNature CommunicationsDecember 1, 2022Xiaoyu Guo, Xue Ding, Juan Dongstandard western blotting analysis and immunodetection with anti-Thiophosphate ester antibody (Abcam, #ab92570). Protein transient expression in Nicotiana benthamiana leaf epidermis and image analysis Agrobacterium

(17) Metabolic control of progenitor cell propagation during Drosophila tracheal remodelingNature CommunicationsDecember 1, 2022Yue Li et al.min. Immunoblotting was conducted using anti-Thiophosphate ester antibody (rabbit, 1:5000, Abcam, #ab92570) or phospho-specific antibody (rabbit, 1:1000, Cell Signaling Technology, #9920) to determine the kinase

(18) ARID1A loss induces polymorphonuclear myeloid-derived suppressor cell chemotaxis and promotes prostate cancer progressionNature communicationsNovember 26, 2022Li N, Liu Q, Han Y, Pei S, Cheng B, Xu J, Miao X, Pan Q, Wang H, Guo J, et al.ARID1A was analyzed by western blotting with a thiophosphate ester rabbit monoclonal antibody (Abcam; ab92570). Full scan blots, see the Source Data file. Gel filtration Gel-filtration chromatography analyses

(19) Ubiquitination of SARS-CoV-2 NSP6 and ORF7a Facilitates NF-κB ActivationmBioAugust 30, 2022Hironori Nishitsuji, Satoko Iwahori, Mariko Ohmori, Kunitada Shimotohno, Takayuki Murataantibody (1:2,000; catalog no. 2624; CST), anti-thiophosphate ester antibody (1:2,000; catalog no. ab92570; Abcam), and HRP-conjugated anti-rabbit IgG (1:5,000; catalog no. AP187P; Sigma-Aldrich). Anti-HA magnetic

(20) Cdc14 spatiotemporally dephosphorylates Atg13 to activate autophagy during meiotic divisionsJournal of Cell BiologyMay 2, 2022Wenzhi Feng, Orlando ArgÃ¼ello-Miranda, Suhong Qian, Fei Wang(Bio-Rad). We used the following antibodies: recombinant monoclonal anti-thiophosphate ester rabbit IgG [51-8] (ab92570; Abcam), monoclonal anti-GFP mouse IgG (11814460001; 1:5,000; Roche), polyclonal anti-hexokinase 1 rabbit

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Anti-Thiophosphate ester antibody [51-8] from Abcam

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Anti-Thiophosphate ester antibody [51-8] from Abcam

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